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This method includes the polymerization chain reaction (PCR). With the help of this reaction, it is possible to identify the pathogen in a blood test, sputum, saliva, urine, the contents of vesicles, and cerebrospinal fluid. DNA is separated from the material obtained from the patient. Then the fragments specific for this virus are repeatedly copied and the results are recorded. This study is the method of choice due to its high accuracy. PCR is able to distinguish between HSV-1 and HSV-2 and determine the amount of herpes simplex virus, which makes it possible to use this method both for diagnosis and for evaluating the effectiveness of treatment.

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If there is even a minimal amount of HSV in the obtained material, the reaction becomes positive, in the absence - negative. Serological method. Used more often than others. As a research material, blood serum is mainly taken. Diagnosis is based on the detection of antigens (specific viral proteins) and antibodies (specific immune complexes of the body) to the herpes simplex virus. Antibodies are protein complexes that are produced by blood cells. When a pathogen enters the body, antibodies bind to it and activate it after a while.

In HSV disease, the focus is on three types of hydrochlorothiazide. M, G to early proteins and G to late proteins. Antibody M appears in the blood a week after the infection with the herpes virus and indicates an acute, first-time infection. In some people, this protein may be found when an old infection recurs. Antibody G - an indicator of a chronic disease, appears in the body 14-21 days after the disease. Its different concentrations indicate either the transition of the disease to the chronic stage, or the low resistance of the body, or recovery.

 

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  • The serological method allows you to determine the amount of virus and controlto increase its titers in the blood, which makes it possible to evaluate the effectiveness of treatment.
  • For this, sera taken at intervals of 7-14 days are examined. This diagnostic method is based on RNIF and ELISA.
  • The indirect immunofluorescence test (IRIF) is a highly sensitive and specific method. It is based on the binding of microzide + antibody complexes and the subsequent attachment of fluorochrome-labeled antibodies specific to specific antibodies to the antigens of herpes viruses.
  • Subsequently, when glowing with ultraviolet light, complexes are determined that can be counted. Enzyme immunoassay (ELISA) has high accuracy and specificity, about microzide pills.
  • For the diagnosis of HSV, two methods of conducting ELISA are used. labeled antigen and labeled antibody.

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The titer of antibodies in the blood is judged by the brightness of microzide online. The reaction with labeled antibody is more difficult. Labeled antibodies are added after an unlabeled antigen+antibody substrate has already formed. In this case, a new complex is formed, where the antigen is surrounded by two antibodies. This arrangement improves the quality of the ELISA reaction, which helps to detect antibodies even at their low content.

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In the labeled antigen assay, a herpes antigen with a special label is added to buy hydrochlorothiazide online. If there were antibodies in the serum, antigen+antibody complexes are formed. After that, the tools are washed and specific enzymes are added to them that can react with these complexes. Then the reaction takes place and the samples are stained. If the analysis is positive for antibodies M, G to primary proteins and G to secondary proteins, this indicates an initial acute form of the disease.

If the analysis is negative for these types of antibodies, the person has never had a herpes simplex virus. If the test is positive for antibodies M and negative for antibodies G to primary proteins and G to secondary proteins, it can be concluded that the disease has arisen more recently.

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If the analysis is negative for antibodies M and positive for antibodies G to primary proteins and G to secondary proteins - either the second half of the initial acute infection, or an exacerbation (relapse) of herpes disease. If the analysis for M antibodies and G antibodies to primary proteins is negative, and G antibodies to secondary proteins are positive, a strong immunity against the herpes simplex virus has been developed.

Before Your First Visit

The disease can be judged by the percentage of G antibodies. The presence of G antibodies of more than 60% indicates that the person is a carrier of the infection, and the disease has passed into the chronic stage.